Publication details
PPARγ Agonistic Activity of Mimulone and Diplacone Encapsulated in Liposomes and Cyclodextrin Complexes
| Authors | |
|---|---|
| Year of publication | 2025 |
| Type | Article in Periodical |
| Magazine / Source | CHEMISTRYOPEN |
| MU Faculty or unit | |
| Citation | |
| web | https://chemistry-europe.onlinelibrary.wiley.com/doi/pdf/10.1002/open.202500209?getft_integrator=clarivate&utm_source=clarivate |
| Doi | https://doi.org/10.1002/open.202500209 |
| Keywords | cyclodextrins; geranylated flavanones; liposomes; nanoparticles; peroxisome proliferator-activated receptor gamma |
| Description | The therapeutic application of flavonoids is limited by their low solubility, bioavailability, and metabolic stability. This study evaluates the peroxisome proliferator-activated receptor gamma (PPAR gamma) agonistic activity of two geranylated flavonoids from Paulownia tomentosa, mimulone and diplacone, and compares the efficacy of different nanoparticle delivery systems, including liposomes and cyclodextrins, in preserving their biological activity. Using the PPAR gamma CALUX reporter gene assay, it is shown that mimulone dissolved in DMSO and incubated with cell culture activates the PPAR gamma pathway, resulting in 2.97-fold and 3.9-fold increases in luciferase activity at concentrations of 5 and 2.5 mu M, respectively. Diplacone, however, shows significant cytotoxicity, with an average cell viability of about 10% at 10 mu M. Encapsulation in anionic, cationic, and neutral liposomes results in a significant reduction of biological activity of both flavonoids, with the best formulation (anionic liposomes) preserving only 54% of mimulone's activity. In contrast, hydroxypropyl-beta-cyclodextrins (HP-beta-CDs) retain up to 91.5% of mimulone's biological activity and significantly improve the viability profile of diplacone, maintaining cell viability at approximate to 100%. The performance of the HP-beta-CDs can be attributed to their ability to form stable inclusion complexes with hydrophobic molecules. These results suggest that cyclodextrin-based delivery systems might effectively address solubility and stability challenges associated with flavonoid therapy. |
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