Publication details
ADSORPTIVE STRIPPING ANALYSIS OF DAMAGE DNA WITH ADMITTANCE MEASUREMENTS
| Authors | |
|---|---|
| Year of publication | 2000 |
| Type | Article in Proceedings |
| Conference | Book of Abstracts, J.Heyrovský Memorial Symposium, Praha |
| MU Faculty or unit | |
| Citation | |
| Field | Biophysics |
| Keywords | DNA adsorption; electrode double layer admittance; stripping analysis |
| Description | Native, denatured and damaged calf thymus DNA was studied by admittance measurements using hanging mercury drop electrode. We used adsorptive stripping technique with application of forward (negative-going) and reverse (positive-going) potential scan. The effect of alternating current voltage, frequency, adsorption potential, voltage amplitude and adsorption time was studied. Native double-stranded (ds) DNA produced peak 3 and inflexion 2 on the forward but not on the reverse curves. Irradiation of ds DNA with relatively small doses of g-radiation (up to 80 Gy) resulted in an appearance of peak 3 and 2 on reverse voltammetric curves. Similar peaks were observed when ds DNA was sonicated. These results suggest that in damaged regions of ds DNA bases are accessible for the interaction with the electrode producing peak 3 without passing through the potential region U around -1.2 V, where ds DNA is supposed to be slowly opened. |
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