Publication details

Degradation of nitroaromatics - screening method of automatic determination of degradation profile

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Authors

KOTOUČKOVÁ Ludmila NEČA Jiří TEPLÁ Radka NAVRÁTILOVÁ Jitka NĚMEC Miroslav

Year of publication 2002
Type Article in Proceedings
Conference 12th internationl biodeterioration and biodegradation symposium (bisosorption and biodremediaton III )
MU Faculty or unit

Faculty of Science

Citation
Field Microbiology, virology
Keywords BIOSCREEN C; nitroaromatic compounds; degradation
Description The method of determination of degradation profile of colored nitroaromatic compounds by the use of automatic spectrophotometer BIOSCREEN C was developed and used for assessment of degradation of selected nitrophenolic compounds (4-nitrophenol, 4-nitroquaiacol, 4-nitrocatechol). Bacterial strains isolated from soil were used for degradation. Bacterial culture was cultivated in separated cells of the microplate under defined conditions. Degradation profiles for selected strains were confirmed by simultaneous measurement by conventional way - in flasks with 50 ml of media, concentrations of substrates in samples were determined by HPLC.
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