Publication details

Photochemical properties of a potential photosensitiser indocyanine green in vitro

Authors

SKŘIVANOVÁ Kateřina ŠKORPÍKOVÁ Jiřina ŠVIHÁLEK Jiří MORNSTEIN Vojtěch JANISCH Roman

Year of publication 2006
Type Article in Periodical
Magazine / Source Journal of Photochemistry and Photobiology B: biology
MU Faculty or unit

Faculty of Medicine

Citation
Field Biophysics
Keywords Indocyanine green; Photosensitiser; HeLa cells; V79 Chinese hamster fibroblasts; Hydrogen peroxide
Description The study investigates the photochemical properties of a potential photosensitiser, indocyanine green (ICG), in vitro. Cytotoxicity ICG was evaluated on HeLa cells and V79 Chinese hamster fibroblasts by means of MTT assay. HeLa proliferation was studied under a combined influence of effective concentration of 24 – 94 uM ICG and a laser irradiation at different energy densities. Phototoxicity was evaluated at 1, 24, and 48 hours after irradiation. No phototoxic effect was detected 1 hour after irradiation. The maximal phototoxic effect of ICG on HeLa cells was detected for an ICG concentration of 94 uM, a laser output of 360 mW, and the energy density of 99 J/cm2 24 hours after irradiation. ICG and tested by itself non-toxic 20 uM H2O2 were added at the same time to HeLa cells to enhance the phototoxic effects. 48 hours after laser irradiation a significant difference was found between the toxicity of ICG plus peroxide as compared to ICG alone. Addition of H2O2 (20 uM) causes the significant increase of phototoxicity of ICG. The results confirm that ICG could be a suitable substance for photodynamic therapy and we can potentiate its phototoxicity by addition of oxidative agent.
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