Vinca alkaloid effect on microtubules of HeLa cells.
|Year of publication||2006|
|Type||Article in Periodical|
|Magazine / Source||Scripta Medica|
|MU Faculty or unit|
|Keywords||Cytoskeleton; Microtubule disruption; Cytoskeleton recovery; vincristine; vinblastine|
|Description||The study evaluated the effect of selected vinca alkaloids on the microtubule network of heLa cells and the recovery of its disruption. Model experiments were used for demonstration of the quantification of microtubule network changes induced by vinca alkaloids using computer-assisted image analysis. Differences in the arrangement of microtubules were assessed by means of a software for quantification of the cytoskeleton changes in cells treated with vincristine at a concentration of 0,020 g/l and in untreated control cells. The microtubule network recovery process in time intervals of 6, 7, 8, 9, and 10 hours was quantified after treatment with vincristine at a concentration of 0,020 g/l for 60 min using the software for quantification of the cytoskeleton changes.|