Capillary electrophoresis with laser-induced fluorescence detection in analysis of amino acids, paptides and proteins

• Authors: RYVOLOVÁ Markéta; PREISLER Jan; KRÁSENSKÝ Pavel; PŘIKRYL Jan; VRÁBEL Patrik; TÁBORSKÝ Petr
• Year of publication: 2007
• Type: Article in Proceedings
• Conference: 13th International symposium on separation sciences and 13th Advances and Applications of Chromatography in Industry
• MU Faculty or unit: Faculty of Science
• Field: Analytic chemistry
• Keywords: capillary electrophoresis; LIF; proteins
• Description: Laser induced fluorescence (LIF) detection is invaluable especially for unparalleled sensitivity; LIF detector combined with a micro column separation method can be used to detect extremely low amounts of fluorescent analytes, in some cases at single molecule level. Diferent methods of detection in CE LIF (indirect detection, derivatization,..) are summarized.

Related projects

• Molecular basis of cell and tissue regulations
• Centre of Biophysical Chemistry, Bioelectrochemistry and Bioanalysis. New Tools for Genomics, Proteomics and Biomedicine