Publication details

13C detected NMR experiments for measuring chemical shifts and coupling constants in nucleic acid bases


FIALA Radovan SKLENÁŘ Vladimír

Year of publication 2007
Type Article in Periodical
Magazine / Source Journal of Biomolecular NMR
MU Faculty or unit

Faculty of Science

Field Biochemistry
Keywords Chemical shift Direct carbon detection NMR Nucleic acid Quaternary carbon Spin spin coupling
Description The paper presents a set of two dimensional experiments that utilize direct 13C detection to provide proton carbon, carbon carbon and carbon nitrogen correlations in the bases of nucleic acids. The set includes a 13C detected proton carbon correlation experiment for the measurement of 13C 13C couplings, the CaCb experiment for correlating two quaternary carbons, the HCaCb experiment for the 13C 13C correlations in cases where one of the carbons has a proton attached, the HCC TOCSY experiment for correlating a proton with a network of coupled carbons, and a 13C detected 13C 15N correlation experiment for detecting the nitrogen nuclei that cannot be detected via protons. The IPAP procedure is used for extracting the carbon carbon couplings or carbon decoupling in the direct dimension, while the S3E procedure is preferred in the indirect dimension of the carbon nitrogen experiment to obtain the value of the coupling constant. The experiments supply accurate values of 13C and 15N chemical shifts and carbon carbon and carbon nitrogen coupling constants. These values can help to reveal structural features of nucleic acids either directly or via induced changes when the sample is dissolved in oriented media.
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