Determination of isoflavones using liquid chromatography with electrochemical detection

• Authors: MIKELOVÁ Radka; HODEK Petr; HANUŠTIAK Pavel; ADAM Vojtěch; KŘÍŽKOVÁ Soňa; HAVEL Ladislav; STIBOROVÁ Marie; HORNA Aleš; BEKLOVÁ Miroslava; TRNKOVÁ Libuše; KIZEK René
• Year of publication: 2007
• Type: Article in Periodical
• Magazine / Source: Acta Chimica Slovenica
• MU Faculty or unit: Faculty of Science
• Field: Electrochemistry
• Keywords: Isoflavonoids; injection flow analysis; HPLC
• Description: Among the biologically important roles of isoflavones is also their effect on carcinogenesis. We used flow injection analysis and high performance liquid W with electrochemical detection to simultaneously determine certain isoflavones (biochanin A, formononetin, sissotrin, daidzin, daidzein, glycitin, glycitein and genistein). The most suitable chromatographic conditions were: mobile phase: 0.2 mol L-1 acetate buffer (pH 5.0); flow rate 2.0 mL min-1; column and detector temperature: 26 dg. C; detection potential: 800 mV. Under the optimal conditions, the detection limits were in the range of several ng mL-1. Their simultaneous determination takes 15 min.

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