Publication details

Mutagenesis Is Elevated in Male Germ Cells Obtained from DNA Polymerase-beta Heterozygous Mice


ALLEN Diwi HERBERT Damon, C. MCMAHAN Alex, C. ROTREKL Vladimír SOBOL Robert, W. WILSON Samuel, H. WALTER Christi, A.

Year of publication 2008
Type Article in Periodical
Magazine / Source Biology of Reproduction
MU Faculty or unit

Faculty of Medicine

Field Biochemistry
Keywords base excision repair DNA repair gamete biology gametogenesis lacI mutagenesis POLB spermatogenesis spermatogenic cells
Description Gametes carry the DNA that will direct the development of the next generation. By compromising genetic integrity, DNA damage and mutagenesis threaten the ability of gametes to fulfill their biological function.High base excision repair (BER) activity is documented for spermatogenic cells. In vitro short-patch BER activity assays revealed that spermatogenic cell nuclear extracts obtained from Polb heterozygous mice had one third the BER activity of age-matched control mice. The spontaneous mutant frequency for mixed spermatogenic cells prepared from Polb heterozygous mice was 2-fold greater than that of wild-type controls, but no significant effect was found among the somatic tissues tested. These results demonstrate that normal POLB abundance is necessary for normal BER activity, which is critical in maintaining a low germline mutant frequency. Notably, spermatogenic cells respond differently than somatic cells to Polb haploinsufficiency.

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