Publication details
Characteristics of Panton - Valentine leukocidin positive <I>Staphylococcus aureus</I> strains isolated in the Czech Republic
| Authors | |
|---|---|
| Year of publication | 2008 |
| Type | Article in Proceedings |
| Conference | 13th International Symposium on Staphylococci and Staphylococcal Infections |
| MU Faculty or unit | |
| Citation | |
| Web | http://www.isssi2008.com/ |
| Field | Genetics and molecular biology |
| Keywords | Staphylococcus aureus; Panton - Valentine leukocidin; MRSA; community acquired infection |
| Description | Objectives: Phenotypic and genotypic characteristics of PVL positive S. aureus strains isolated from clinical specimens in the Czech Republic between January 2004 and April 2008 are described. Methods: Carriage of the lukS-PV and lukF-PV genes for PVL, and mecA gene was tested by a multiplex PCR assay. The phenotypic and genotypic methods were used for detection of superantigens (enterotoxin A-J, TSST-1, and exfoliatins A and B). The PVL positive MRSA strains were characterized in detail by PFGE, spa typing, SCCmec typing, arcA gene detection, agr typing, plasmid typing, and prophage typing. Results: The presence of the lukS-PV and lukF-PV genes was detected in 159 (6.8 %) of 2337 S. aureus strains. Only 21 strains were MRSA, 17 of them were SCCmec type IV, three SCCmec V and one harbored a composite SCCmec with ccrB and ccrC recombinases. Genotypic properties of PVL positive MRSA strains were correlated with the known European caMRSA types. In addition to the predominant spa-type t008 (38 %) exhibiting the characteristics of USA300 clone and t044 (14 %) widely disseminated in Central Europe, other spa-types were sporadically represented by t002, t003, t186, t311, t433 and t667 among the caMRSA isolates. Conclusions: We found 159 PVL positive MRSA and MSSA isolates, mainly from lesions, wound infection, skin and soft tissue abscesses, and abscessing pneumonia. Four pneumonia cases were fatal, three causative strains were MRSA, with two of them related to clone USA300. Most (79 %) PVL positive strains were phage typeable and were predominantly classified into phage group II (52 %). The lukS-PV and lukF-PV genes were in all analysed strains associated with the Sa2 type prophages of serogroups A or F. |
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