Publication details

A complex role for FGF-2 in self renewal, survival, and adhesion of human embryonic stem cells

Authors

EISELLEOVÁ Lívia MATULKA Kamil KŘÍŽ Vítězslav KUNOVÁ Michaela SCHMIDTOVÁ Zuzana NERADIL Jakub TICHÝ Boris DVOŘÁKOVÁ Dana POSPÍŠILOVÁ Šárka HAMPL Aleš DVOŘÁK Petr

Year of publication 2009
Type Article in Periodical
Magazine / Source Stem Cells
MU Faculty or unit

Faculty of Medicine

Citation
Field Genetics and molecular biology
Keywords FGF-2; human ESCs; self renewal; cell survival; adhesion
Description The transcription program that is responsible for the pluripotency of human ESCs (hESCs) is believed to be comaintained by exogenous fibroblast growth factor-2 (FGF-2), which activates FGF receptors (FGFRs) and stimulates the mitogen-activated protein kinase (MAPK) pathway. This mechanism is further complicated by intracrine FGF signals.Here we show that, in undifferentiated hESCs, exogenous FGF-2 and inhibition of autocrine FGF signaling stimulated the expression of stem cell genes while suppressing cell death and apoptosis genes. Thus, exogenous FGF-2 reinforced the pluripotency maintenance program of intracrine FGF-2 signaling. Consistent with this hypothesis, expression of endogenous FGF-2 decreased during hESC differentiation and FGF-2 knockdown-induced hESC differentiation. In addition, FGF-2 signaling via FGFR2 activated MAPK kinase/extracellular signal-regulated kinase and AKT kinases, protected hESC from stress-induced cell death, and increased hESC adhesion and cloning efficiency.

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