Publication details

Avoidance tests of solid waste samples with Enchytraeus albidus, Enchytraeus crypticus and Eisenia fetida



Year of publication 2009
Type Conference abstract
MU Faculty or unit

Faculty of Science

Description Contact bioassays are important for testing ecotoxicity of solid materials. However, survival and reproduction tests are often not practical due to their duration (up to several weeks). Moreover, mortality as an endpoint is robust parameter not useful as an early warning. From these reasons, the avoidance tests with soil invertebrates suggest good alternative or extension to the classic test batteries. They offer very short time duration (days) and sensitive subacute endpoint (behavior). The aim of our study was to compare sensitivity of three oligochaete species (enchytraeids Enchytraeus albidus, Enchytraeus crypticus and earthworm Eisenia fetida) in avoidance tests of three types of wastes. Incineration ash, contaminated soil and contaminated wood chips were diluted with artificial soil in scaled ratios and clear artificial soil was used as control. The avoidance tests lasted 2 days and they were designed as two-chamber test systems. The toxicity of wastes decreased in this order: wood > ash > soil for all used species. E. fetida was the most sensitive species and E. crypticus the least sensitive one (E. fetida: EC50ash < 3.13%, EC50wood < 4.5%, EC50soil < 25%; E. albidus: EC50ash = 4.42%, EC50wood = 9.55%, EC50soil > 100%; E. crypticus: EC50ash = 14.33%, EC50wood = 9.17%, EC50soil > 100%). Our results indicate that avoidance tests with earthworms and enchytraeids are feasible for waste testing
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