Publication details
Improved sensitivity and selectivity of uric acid voltammetric sensing with mechanically grinded carbon/graphite electrodes
| Authors | |
|---|---|
| Year of publication | 2009 |
| Type | Article in Periodical |
| Magazine / Source | Electrochimica Acta |
| MU Faculty or unit | |
| Citation | |
| Field | Biophysics |
| Keywords | Grinded carbon electrodes; Voltammetry; Uric acid; Dopamine; Hypoxanthine; Xanthine; Adenine; Guanine; Purine metabolism; Diagnostics |
| Description | In this paper we show that a strong enhancement of the UA oxidation signal can be reached by a simple mechanical grinding of the surfaces of glassy carbon or edge plane-oriented pyrolytic graphite electrodes with SiC particles of an optimum size 15 um in contrast to fine polished electrodes. The grinded ones exhibited an excellent separation of oxidation signals of ascorbic acid, dopamine, xanthine and hypoxanthine, making it possible to detect these substances in a mixture. Enhancement of UA and dopamine (DA) oxidation signals at the grinded electrodes allowed their easy detection at nanomolar levels in up to 10e4-fold excesses of ascorbic acid. Due to a strong adsorption at the electrode surface, nanomolar concentrations of UA and DA can be determined by ex situ voltammetry. Similarly strong enhancement of oxidation signals was observed for purine nucleobases, guanine and adenine. The grinded electrodes have been tested in analysis of real clinical samples of human serum or urine. |