Mosquito (Diptera: Culicidae) surveillance for arboviruses in an area endemic for West Nile (lineage Rabensburg) and Ťahyňa viruses in Central Europe.
|Year of publication||2010|
|Type||Article in Periodical|
|Magazine / Source||Journal of Medical Entomology|
|MU Faculty or unit|
|Keywords||Ťahyňa virus; West Nile virus; Aedes vexans; Aedes rossicus|
|Description||Six viral isolates were obtained from 23,243 female mosquitoes belonging to 16 species and collected in South Moravia (Czechland) during 2006 through 2008: five isolates of Orthobunyavirus Ťahyňa (TAHV, California group, family Bunyaviridae): 3 isolations from Aedes vexans, 1 from Ae. sticticus, 1 from Culex modestus), and one isolation of Flavivirus West Nile (WNV, Japanese encephalitis group, family Flaviviridae; strain Rabensburg lineage 3 of WNV) from Aedes rossicus. All viral isolates were recovered from mosquitoes collected in 2006 (15,882 mosquitoes examined), while no virus was isolated from mosquitoes trapped in 2007 and 2008, when 1,555 and 5,806 mosquitoes were examined, respectively, and the population density was very low due to warm and dry summer including a considerably low water table, compared to environmental conditions favorable for mosquito development in 2006. The virus isolation procedure was based on intracerebral inoculation of newborn mice. In parallel, 8470 mosquitoes were also examined by inoculating Vero cell cultures. The latter method detected only 3 of the 6 virus isolates (including WNV). Aedes rossicus is a new potential vector for WNV.|