Publication details

In vitro activation of CMV-specific human CD8+ T cells by CyaA toxoids delivering pp65 epitopes



Year of publication 2010
Type Conference abstract
MU Faculty or unit

Faculty of Medicine

Description In this study, we investigated the capacity of two CyaA constructs to deliver epitopes from the CMV phosphoprotein pp65 to activate T cells of CMV-seropositive and CMV-seronegative healthy donors in vitro. Immune response of T cells was monitored using Flow cytometry and Cytometric Beads Array. Expansion of CMV-specific T cells secreting gamma-interferon as well as increase of total IFN-gamma and TNF-alfa; production could be detected after in vitro stimulation with CyaA-CMV constructs, while limited activation of T cells occurred with free peptides. No immune response was evoked by control CyaA without CMV antigenic peptides. These data reveal the potential of CyaA constructs as a tool for fast and reliable detection of CMV-specific immunity and for immunoprophylactic, as well as immunotherapeutic application against CMV-associated diseases in immunocompromised patients.
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