Publication details

Directional motion of foreign plasmid DNA to nuclear HP1 foci

Authors

ONDŘEJ Vladan KOZUBEK Stanislav LUKÁŠOVÁ Emilie FALK Martin MATULA Pavel MATULA Petr KOZUBEK Michal

Year of publication 2006
Type Article in Periodical
Magazine / Source Chromosome Research
MU Faculty or unit

Faculty of Informatics

Citation
Field Genetics and molecular biology
Keywords heterochromatin; HP1 protein; nuclear compartments; plasmid; transfection
Description Movement of labelled plasmid DNA relative to heterochromatin foci in nuclei, visualized with HP1-GFP, was studied using live-cell imaging and object tracking. In addition to Brownian motion of plasmid DNA we found a pronounced, non-random movement of plasmid DNA towards the nearest HP1 focus, while time-lapse microscopy showed that HP1 foci are relatively immobile and positionally stable. The movement of plasmid DNA was much faster than that of the HP1 foci. Contact of transgene DNA with an HP1 focus usually resulted in cessation of the directional motion. Moreover, the motion of plasmid DNA inside the heterochromatin compartment was more restricted (limited to 0.25 mu m) than when the plasmid DNA was outside heterochromatin (R = 0.7 mu m). Three days after transfection most of the foreign labelled DNA colocalized with centromeric heterochromatin.
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