Genome analysis of Treponema pallidum subsp. pallidum and subsp. pertenue strains by whole genome fingerprinting: most of the genetic differences are localized in six genomic regions.
|Year of publication||2010|
|Type||Article in Periodical|
|Magazine / Source||PloS ONE|
|MU Faculty or unit|
|Field||Genetics and molecular biology|
|Keywords||Treponema pallidum subsp. pallidum; Treponema pallidum subsp. pertenue; syphilis; yaws;|
|Description||The genomes of eight treponemes including T. p. pallidum strains (Nichols, SS14, DAL1 and Mexico A), T. p. pertenue strains (Samoa D, CDC2 and Gauthier), and the FribourgBlanc isolate, were amplified in 133 overlapping amplicons, and the restriction patterns of these fragments were compared. The approximate genome sequence identity investigated based on this whole genome fingerprinting (WGF) analysis ranged from 99.57 to 99.98% in the homologous genome regions. Restriction target site analysis, detecting the presence of 1773 individual restriction sites found in the reference Nichols genome, revealed a high genome structure similarity of all strains. Most of the genetic differences between T. p. pallidum and T. p. pertenue strains were accumulated in six genomic regions. These genome differences likely contribute to the observed differences in pathogenicity between T. p. pallidum and T. p. pertenue strains and could be used for the molecular detection.|