Publication details

Sex determination in children and undeterminable skeletal remains from burial site Pohansko near Breclav



Year of publication 2009
Type Appeared in Conference without Proceedings
MU Faculty or unit

Faculty of Science

Description The archaeological area Pohansko is situated in the south-east area of the Czech Republic, near the town Breclav. The South Outer Precinct is the second largest burial site that was discovered at Pohansko. The skeletal remains from The South Outer Precinct are badly preserved, the bones are fragmentary and in most cases unidentifiable. Of the 190 skeletons, only 27 were determined as male, 40 as female and 87 as children. The sex of the remaining 36 skeletons was impossible to define by standard methods. The aim of this study was to determine the sex of childrens and undeterminable skeletons using two sex markers – the amelogenin gene and the SRY gene. The amelogenin gene shows size differences between the X and the Y human chromosome. We amplified short fragments of the amelogenin gene, 106 bp of the X chromosome and 112 bp of the Y chromosome. A short 93 bp fragment of the male specific SRY gene was used as the second marker. Bone material was excavated during a rescue archaeological research in the period of 1975 – 1979. The results are important for demography of the burial site.
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