Publication details

Genetická studie dětí z lokality Pohansko - Jižní předhradí

Title in English Genetic study of children from Pohansko - The South Outer Precinct


Year of publication 2006
Type Appeared in Conference without Proceedings
MU Faculty or unit

Faculty of Science

Description The South Ramparts burial ground is the second largest burial ground discovered in Pohansko. Some 210 skeletal graves have been excavated there, from which the remains of 189 people have been taken. Unfortunately, the skeletal finds from South Ramparts are rather badly preserved, and in most cases unidentifiable. Of the 189 skeletons, only 27 were determined as adult male, 40 as adult female and 88 as children; the sex of the remaining 34 skeletons was impossible to define by standard morphometric and morphological methods. Polymerase chain reaction (PCR) is often the only way to analyse the DNA from ancient skeletal finds containing only a tiny proportion of DNA, or in which the DNA is partially degraded. Such molecular-biological procedures do not concentrate on compltete chromosomes but only on certain loci within the DNA. The PCR method enables the determination of the sex of the sample originator by several means. The most frequent procedures include an analysis of the SRY gene and the amelogenin gene. These methods are also employed to determine the sex of childrens skeletal remains from South Ramparts. The information thus acquired is vital to the demography of the entire burial ground.
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