Informace o publikaci

Genotypic characterization of four major clonal lineages of impetigo Staphylococcus aureus strains

Logo poskytovatele


Rok publikování 2012
Druh Konferenční abstrakty
Fakulta / Pracoviště MU

Přírodovědecká fakulta

Popis OBJECTIVE: We aimed at detailed genotypic investigations of a collection of 127 exfoliative toxin producing S. aureus strains most of which caused neonatal skin blistering disorders in 23 geographically distant maternity hospitals in the Czech Republic and in one Slovak hospital in 1998 - 2011. METHODS: The eta, etb and etd genes, as well as presence of four serotypes of prophages (A, B, Fa, and Fb) were detected by a multiplex PCR amplification. ETA and ETB positive isolates were characterised by Multilocus sequence typing (MLST), pulsed-field gel electrophoresis (PFGE), spa typing and ETA-B prophage carriage. RESULTS: Ninety-eight strains isolated from children, three of them were identified as a source of acute SSSS. The remaining 29 isolates originated from human carriers and/or hospital equipment. In a collection of 127 strains we identified 21 genotypes grouped into four clonal complexes (CC). The predominant CC121 including 47 strains has occurred in 13 Czech hospitals. Thirty-nine CC15 strains were classified into three sequence types (ST15, ST582, and ST2195). Among 24 CC9 strains, from which 9 were isolates from persons with nasal carriage, ST9 (7 isolates), ST109 (11 strains), and novel ST2194 (6 isolates) were determined. Seventeen strains showing similar PFGE fingerprints were assigned to ST88. The lineages CC9, CC15 and CC88 exclusively included eta gene-positive strains while the strains belonging to CC121 harboured the eta and/or etb genes. The ETB producing strains belonged entirely to CC121. CONCLUSION: Four major lineages of ET-positive strains are associated with impetigo skin disorders in young children in the Czech Republic. This study highlights the significant genomic diversity among impetigo S. aureus strains and the distribution of major genotypes disseminated in the Czech and Slovak maternity hospitals.
Související projekty:

Používáte starou verzi internetového prohlížeče. Doporučujeme aktualizovat Váš prohlížeč na nejnovější verzi.

Další info