Informace o publikaci

TGF-beta 1 signaling plays a dominant role in the crosstalk between TGF-beta 1 and the aryl hydrocarbon receptor ligand in prostate epithelial cells

Autoři	STARŠÍCHOVÁ Andrea HRUBÁ Eva SLABÁKOVÁ Eva PERNICOVÁ Zuzana PROCHÁZKOVÁ Jiřina PĚNČÍKOVÁ Kateřina ŠEDA Václav KABÁTKOVÁ Markéta VONDRÁČEK Jan KOZUBÍK Alois MACHALA Miroslav SOUČEK Karel
Rok publikování	2012
Druh	Článek v odborném periodiku
Časopis / Zdroj	Cellular Signalling
Fakulta / Pracoviště MU	Přírodovědecká fakulta
Citace
Doi	http://dx.doi.org/10.1016/j.cellsig.2012.04.008
Obor	Genetika a molekulární biologie
Klíčová slova	Transforming growth factor-beta; Prostate epithelial cells; Aryl hydrocarbon receptor; SMAD4
Popis	Crosstalk between the aryl hydrocarbon receptor (AhR) and transforming growth factor-beta 1 (TGF-beta 1) signaling has been observed in various experimental models. However, both molecular mechanism underlying this crosstalk and tissue-specific context of this interaction are still only partially understood. In a model of human non-tumorigenic prostate epithelial cells BPH-1, derived from the benign prostatic hyperplasia, 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) persistently activates the AhR signaling pathway and induces expression of xenobiotic metabolizing enzymes, such as CYP1A1 or CYP1B1. Here we demonstrate that TGF-beta 1 suppresses the AhR-mediated gene expression through multiple mechanisms, involving inhibition of AhR expression and down-regulation of nuclear AhR, via a SMAD4-dependent pathway. In contrast, TCDD-induced AhR signaling does not affect either TGF-beta 1-regulated gene expression or epithelial-to-mesenchymal transition. These observations suggest that, in the context of prostate epithelium, TGF-beta 1 signaling plays a dominant role in the crosstalk with AhR signaling pathway. Given the importance of TGF-beta 1 signaling in regulation of prostate epithelial tissue homeostasis, as well as the recently revealed role of AhR in prostate development and tumorigenesis, the above findings contribute to our understanding of the mechanisms underlying the crosstalk between the two signaling pathways in the prostate-specific context. (C) 2012 Elsevier Inc. All rights reserved.