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Pseudomonas prosekii sp. nov., a Novel Psychrotrophic Bacterium from Antarctica

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KOSINA Marcel BARTÁK Miloš MAŠLAŇOVÁ Ivana VÁVROVÁ PASCUTTI Andrea ŠEDO Ondrej LEXA Matej SEDLÁČEK Ivo

Rok publikování 2013
Druh Článek v odborném periodiku
Časopis / Zdroj Current Microbiology
Fakulta / Pracoviště MU

Přírodovědecká fakulta

Citace
www http://link.springer.com/article/10.1007%2Fs00284-013-0406-6
Doi http://dx.doi.org/10.1007/s00284-013-0406-6
Obor Mikrobiologie, virologie
Klíčová slova Antarctic; Pseudomonas; Taxonomy; rpoB; rrs; rpoD
Přiložené soubory
Popis During Czech expeditions at James Ross Island, Antarctica, in the years 2007–2009, the bacterial diversity of the genus Pseudomonas was studied. Twelve fluorescent Pseudomonas strains were isolated from various samples and were subjected to a detailed taxonomic study. A polyphasic approach included genotypic and phenotypic analyses. The genotypic analysis involved sequencing of rrs, rpoB and rpoD genes, DNA–DNA hybridization (DDH) studies as well as manual ribotyping using HindIII endonuclease. The phenotypic characterization included conventional tests as well as biotyping using the Biolog system, protein profiling by SDS-PAGE, and MALDI-TOF MS analysis. Our taxonomic study revealed that all isolates belonged to the same Pseudomonas species with psychrotrophic growth not exceeding 37C. The cultures showed a unique position among the phylogenetically related pseudomonads. DDH experiment between the proposed type strain of the antarctic isolates and the closest neighbour P. arsenicoxydans CCM 8423T showed only 40.9–50.1 % similarity, thus confirming that the characterized strains do not belong to the P. arsenicoxydans species. According to the results obtained we propose the name P. prosekii sp. nov. for this novel Pseudomonas taxon with type strain AN/28/1T (=CCM 7990T and LMG 26867T).
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