Informace o publikaci

Association of genetic variability in selected regions in visfatin (NAMPT) gene with anthropometric parameters and dietary composition in obese and non-obese Central-European population

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ŠŤASTNÝ Jiří BIENERTOVÁ VAŠKŮ Julie TOMANDL Josef TOMANDLOVÁ Marie ZLÁMAL Filip FOREJT Martin ŠPLÍCHAL Zbyněk VAŠKŮ Anna

Rok publikování 2013
Druh Článek v odborném periodiku
Časopis / Zdroj Diabetes & metabolic syndrome
Fakulta / Pracoviště MU

Lékařská fakulta

Citace
Doi http://dx.doi.org/10.1016/j.dsx.2013.06.001
Obor Fyziologie
Klíčová slova Anthropometric parameters; Dietary composition; Extreme obesity; NAMPT; SNP; Visfatin
Přiložené soubory
Popis Aims Visfatin (NAMPT/PBEF) is a recently identified adipocytokine which harbors strong insulin-mimetic activity and was reported to be associated with obesity. However, nothing is known about whether visfatin is related to specific nutritional behavior which may result in obesity development. This is the first study focusing on genetic variability of the visfatin gene and its association with circulating visfatin, anthropometric parameters and dietary composition. Materials and Methods We analyzed a total of 11 exons and adjacent non-coding regions of the NAMPT gene in 20 extremely obese Czech individuals (mean BMI 52.2 +/- 5.0 SD) using direct sequencing and a frequency of rs2302559 was established in the validation cohort of another 605 individuals with completed 7-day food records and complex anthropometric measurements. Serum levels of visfatin, leptin and leptin-receptor were measured in all sequenced individuals and in part of the validation cohort. Results Three common polymorphisms were identified, two in non-coding regions (rs78411774 A/C, rs71564769 A/C) and one synonymous SNP in exon 7 (rs2302559 A/G). The rs2302559 showed significant correlation with visfatin serum level throughout the entire study cohort (p < 0.001); there was a significant tendency toward higher visfatin levels in G allele carriers with GG homozygotes having the highest visfatin serum levels. Furthermore, a negative correlation was observed between visfatin and leptin serum level (p = 0.01). No association between investigated SNPs and anthropometric parameters or native dietary composition was observed. Conclusion This is the first study to demonstrate that the rs2302559 polymorphism in the PBEF gene is related to circulating levels of visfatin. As the SNP is synonymous, we hypothesize it might be linked to another SNP in the PBEF gene which controls visfatin serum levels.