Informace o publikaci

DspA from Strongylocentrotus purpuratus: the First Biochemically Characterized Haloalkane Dehalogenase of Non-Microbial Origin.

Autoři

FOŘTOVÁ Andrea ŠEBESTOVÁ Eva ŠTĚPÁNKOVÁ Veronika KOUDELÁKOVÁ Táňa PÁLKOVÁ Lenka DAMBORSKÝ Jiří CHALOUPKOVÁ Radka

Druh Článek v odborném periodiku
Časopis / Zdroj Biochimie
Fakulta / Pracoviště MU

Přírodovědecká fakulta

Citace
Doi http://dx.doi.org/10.1016/j.biochi.2013.07.025
Obor Biochemie
Klíčová slova Haloalkane Dehalogenase
Popis Haloalkane dehalogenases are known as bacterial enzymes cleaving a carbon-halogen bond in halogenated compounds. Here we report the first biochemically characterized non-microbial haloalkane dehalogenase DspA from Strongylocentrotus purpuratus. The enzyme shows a preference for terminally brominated hydrocarbons and enantioselectivity towards beta-brominated alkanes. Moreover, we identified other putative dehalogenases of eukaryotic origin, representing targets for future experiments to discover dehalogenases with novel catalytic properties.
Související projekty: