Informace o publikaci

Arabidopsis TWISTED DWARF1 Functionally Interacts with Auxin Exporter ABCB1 on the Root Plasma Membrane

Autoři	WANG Bangjun BAILLY Aurelien ZWIEWKA Marta HENRICHS Sina AZZARELLO Elisa MANCUSO Stefano MAESHIMA Masayoshi FRIML Jiří SCHULZ Alexander GEISLER Markus
Rok publikování	2013
Druh	Článek v odborném periodiku
Časopis / Zdroj	Plant Cell
Fakulta / Pracoviště MU	Středoevropský technologický institut
Citace
www	http://www.plantcell.org/content/25/1/202
Doi	http://dx.doi.org/10.1105/tpc.112.105999
Obor	Genetika a molekulární biologie
Klíčová slova	BINDING CASSETTE TRANSPORTER; PLANT DEVELOPMENT; P-GLYCOPROTEINS; LATERAL ROOT; EFFLUX; IMMUNOPHILIN; RESISTANCE; CELLS; PIN; LOCALIZATION
Popis	Plant architecture is influenced by the polar, cell-to-cell transport of auxin that is primarily provided and regulated by plasma membrane efflux catalysts of the PIN-FORMED and B family of ABC transporter (ABCB) classes. The latter were shown to require the functionality of the FK506 binding protein42 TWISTED DWARF1 (TWD1), although underlying mechanisms are unclear. By genetic manipulation of TWD1 expression, we show here that TWD1 affects shootward root auxin reflux and, thus, downstream developmental traits, such as epidermal twisting and gravitropism of the root. Using immunological assays, we demonstrate a predominant lateral, mainly outward-facing, plasma membrane location for TWD1 in the root epidermis characterized by the lateral marker ABC transporter G36/PLEIOTROPIC DRUG-RESISTANCE8/PENETRATION3. At these epidermal plasma membrane domains, TWD1 colocalizes with nonpolar ABCB1. In planta bioluminescence resonance energy transfer analysis was used to verify specific ABC transporter B1 (ABCB1)-TWD1 interaction. Our data support a model in which TWD1 promotes lateral ABCB-mediated auxin efflux via protein-protein interaction at the plasma membrane, minimizing reflux from the root apoplast into the cytoplasm.