Informace o publikaci

Lincomycin Biosynthesis Involves a Tyrosine Hydroxylating Heme Protein of an Unusual Enzyme Family

Autoři

NOVOTNÁ Jitka OLSOVSKA Jana NOVAK Petr MOJZEŠ Peter CHALOUPKOVÁ Radka KAMENIK Zdenek SPIZEK Jaroslav KUTEJOVA Eva MAREČKOVÁ Markéta TICHY Pavel DAMBORSKÝ Jiří JANATA Jiri

Rok publikování 2013
Druh Článek v odborném periodiku
Časopis / Zdroj Plos One
Fakulta / Pracoviště MU

Středoevropský technologický institut

Citace
www http://www.plosone.org/article/fetchObject.action?uri=info%3Adoi%2F10.1371%2Fjournal.pone.0079974&representation=PDF
Doi http://dx.doi.org/10.1371/journal.pone.0079974
Obor Biotechnologie a bionika
Klíčová slova HORSERADISH-PEROXIDASE; HETEROLOGOUS EXPRESSION; STABLE ISOTOPES; ACID; CYTOCHROME-P450; PEROXYGENASE; MECHANISMS; CHLOROPEROXIDASE; SIBIROMYCIN; TOMAYMYCIN
Přiložené soubory
Popis The gene lmbB2 of the lincomycin biosynthetic gene cluster of Streptomyces lincolnensis ATCC 25466 was shown to code for an unusual tyrosine hydroxylating enzyme involved in the biosynthetic pathway of this clinically important antibiotic. LmbB2 was expressed in Escherichia coli, purified near to homogeneity and shown to convert tyrosine to 3,4-dihydroxyphenylalanine (DOPA). In contrast to the well-known tyrosine hydroxylases (EC 1.14.16.2) and tyrosinases (EC 1.14.18.1), LmbB2 was identified as a heme protein. Mass spectrometry and Soret band-excited Raman spectroscopy of LmbB2 showed that LmbB2 contains heme b as prosthetic group. The CO-reduced differential absorption spectra of LmbB2 showed that the coordination of Fe was different from that of cytochrome P450 enzymes. LmbB2 exhibits sequence similarity to Orf13 of the anthramycin biosynthetic gene cluster, which has recently been classified as a heme peroxidase. Tyrosine hydroxylating activity of LmbB2 yielding DOPA in the presence of (6R)-5,6,7,8-tetrahydro-L-biopterin (BH4) was also observed. Reaction mechanism of this unique heme peroxidases family is discussed. Also, tyrosine hydroxylation was confirmed as the first step of the amino acid branch of the lincomycin biosynthesis.
Související projekty:

Používáte starou verzi internetového prohlížeče. Doporučujeme aktualizovat Váš prohlížeč na nejnovější verzi.

Další info