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Study of interaction of benzo[c]phenanthridine alkaloids with DNA

Název česky Studium interakce benzo[c]fenanthridinových alkaloidů s DNA
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PAROULEK Petr RÁJECKÝ Michal PĚNČÍKOVÁ Kristýna TÁBORSKÝ Petr

Rok publikování 2014
Druh Konferenční abstrakty
Citace
Popis G-quadruplex (GQ) is one of non-canonical structures of nucleic acids (NA). Structures of intramolecular GQs (formed by only one strand of NA) can be distinguished into two groups according to sequence orientation: parallel and anti-parallel GQs [1]. The effect of selected benzo[c]phenanthridine and protoberberine alkaloids from plants of Papaveraceae family [2] on stability of parallel (sequence from c- kit oncogene promoter) and anti-parallel (human telomeric sequence) GQ was studied. Stabilization (in terms of melting point shifts) of GQs by interaction with macarpine, sanguilutine, sanguinarubine, sanguinarine, chelerythrine and corysamine was followed by UV-vis and CD spectrometry. Results showed that all tested alkaloids except macarpine have a stabilizing effect on human telomeric sequence (?T m = 3-17 °C). Parallel sequence from c-kit oncogene promoter was stabilized by all alkaloids.
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