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Cystatins of the parasitic nematodes from the genus Trichinella

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ŠKORPÍKOVÁ Lucie ŠRÁMOVÁ Eliška ILGOVÁ Jana KOUDELA Břetislav GELNAR Milan KAŠNÝ Martin

Rok publikování 2014
Druh Další prezentace na konferencích
Fakulta / Pracoviště MU

Přírodovědecká fakulta

Citace
Popis The nematodes of the genus Trichinella are intracellular parasites of small intestine and skeletal muscle cells. They can infect a broad range of worldwide distributed hosts like birds, reptiles and mammals. All Trichinella spp. are zoonotic but only six of twelve genotypes have been detected in humans, causing the trichinellosis - a serious, sometimes fatal, human disease, which has been documented in 55 countries of the World. Our study is focused on characterization of composition of excretory-secretory products (ESP) from L1 muscle larvae of three trichinella species - Trichinella spiralis, T. britovi and T. pseudospiralis. The dominant components of L1 ESP are cystatins. These proteins are a reversible inhibitors of proteolytics enzymes - cystein peptideases and they are involved in many relevant biological processes such as development, growth, digestion and migration of parasites and suppression of host immune responses. The main aim of our study is to prepare the recombinant form of three trichinella cystatin and compare/characterize their properties. We used primers specifically designed for identified “multi cystatin-like domain” gene of T. spiralis. Subsequently, we obtained sequences of this gene for the other two species and we compared these data. We found out that cystatin genes of T. spiralis and T. britovi have high sequence homology although gene of T. pseudospiralis differs more (e.g. number of domains, conserved motifs).
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