Informace o publikaci

Coilin is rapidly recruited to UVA-induced DNA lesions and gamma-radiation affects localized movement of Cajal bodies

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BÁRTOVÁ Eva FOLTÁNKOVÁ Veronika LEGARTOVÁ Soňa SEHNALOVÁ Petra SOROKIN Dmitry SUCHÁNKOVÁ Jana KOZUBEK Stanislav

Rok publikování 2014
Druh Článek v odborném periodiku
Časopis / Zdroj Nucleus
Fakulta / Pracoviště MU

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Citace
Doi http://dx.doi.org/10.4161/nucl.29229
Obor Genetika a molekulární biologie
Klíčová slova DNA repair; chromatin; nucleus; nucleolus; Cajal bodies; coilin
Popis Cajal bodies are important nuclear structures containing proteins that preferentially regulate RNA-related metabolism. We investigated the cell-type specific nuclear distribution of Cajal bodies and the level of coilin, a protein of Cajal bodies, in non-irradiated and irradiated human tumor cell lines and embryonic stem (ES) cells. Cajal bodies were localized in different nuclear compartments, including DAPI-poor regions, in the proximity of chromocenters, and adjacent to nucleoli. The number of Cajal bodies per nucleus was cell cycle-dependent, with higher numbers occurring during G2 phase. Human ES cells contained a high coilin level in the nucleoplasm, but coilin-positive Cajal bodies were also identified in nuclei of mouse and human ES cells. Coilin, but not SMN, recognized UVA-induced DNA lesions, which was cell cycle-independent. Treatment with gamma-radiation reduced the localized movement of Cajal bodies in many cell types and GFP-coilin fluorescence recovery after photobleaching was very fast in nucleoplasm in comparison with GFP-coilin recovery in DNA lesions. By contrast, nucleolus-localized coilin displayed very slow fluorescence recovery after photobleaching, which indicates very slow rates of protein diffusion, especially in mouse ES cell nucleoli.
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