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LIQUID CHROMATOGRAPHIC MASS SPECTROMETRIC DETECTION OF METABOLITES OF MINOR BENZO[C]PHENANTHRIDINE ALKALOIDS

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MIDLIK Adam SÁNDOR Roman NOSKOVÁ Kristýna DOVRTĚLOVÁ Gabriela JUŘICA Jan TÁBORSKÁ Eva PEŠ Ondřej

Rok publikování 2014
Druh Článek ve sborníku
Konference CECE 2014: 11TH INTERNATIONAL INTERDISCIPLINARY MEETING ON BIOANALYSIS
Fakulta / Pracoviště MU

Lékařská fakulta

Citace
Obor Biochemie
Klíčová slova benzophenanthridine; metabolite; microsomes; mass spectrometry; liquid chromatography
Přiložené soubory
Popis Quaternary benzo[c]phenanthridine alkaloids (QBAs) belong to a group of substances obtained from plants of families Papaveraceae, Fumariaceae and Rutaceae. While the major and commercially available sanguinarine (SA) and chelerythrine (CHE) have been involved in biological and pharmacological studies over recent years, metabolic data on minor QBAs including pentasubstituted sanguilutine (SL) and hexasubstituted macarpine (MA) are limited, due to their slow accumulation in plant material as they represent the end products in their respective biosynthetic pathways. One of the substantial parts of the pharmacokinetic and toxicity studies is determination of their metabolic fate. Enzymes of endoplasmatic reticulum, located on rat liver microsomes (RLMs), such as cytochrome P450, may serve as a simple in vitro model for metabolic studies. The metabolites can be analyzed by high performance liquid chromatography (HPLC) coupled to tandem mass spectrometry (MS). Despite the very similar structure of QBAs, metabolic pathways may differ considerably. Results published on metabolites of SA and CHE indicate demethylation, hydroxylation and ring-opening reactions.
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