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Immune response of porcine alveolar macrophages to simultaneous infection with porcine reproductive and respiratory syndrome virus and Haemophilus parasuis

Název česky Imunitní odpověď prasečích alveolárních makrofágů na ko-infekci virem prasečího reprodukčního a respiračního syndromu a Haemophilem parasuis
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KAVANOVÁ Lenka PRODĚLALOVÁ Jana NEDBALCOVÁ Kateřina MATIAŠOVIC Ján VOLF Jiří FALDYNA Martin SALÁT Jiří

Druh Konferenční abstrakty
Fakulta / Pracoviště MU

Přírodovědecká fakulta

Citace
Popis Porcine reproductive and respiratory syndrome virus (PRRSV) can predispose pigs to the secondary respiratory infection with bacteria such as Haemophilus parasuis. Animals infected with both pathogens develop more severe clinical disease. Our study was aimed on immune response of porcine alveolar macrophages (PAMs) to simultaneous infection with PRRSV and H. parasuis in vitro, especially cytokine production, the expression of immunologically-relevant surface cell molecules and their functional and antimicrobial characteristics such as respiratory burst. Gene expression was measured by RT-PCR. Simultaneous infection with PRRSV and H. parasuis increased gene expression of pro-inflammatory cytokines (TNF-?, IL-1?, IL-8) in PAMs in comparison with PAMs infected with PRRSV or H. parasuis only, which was confirmed on protein level by ELISA. Synergistic effect of dual infection was also observed on mRNA production of co-stimulatory molecule CD80. This effect was not observed in the case of CD163 and MHCII. Production of reactive oxygen species (ROS) was measured by luminometry. PAMs infected with PRRSV increased production of ROS in comparison with non-infected PAMs. On the contrary, simultaneous infection of PAMs with PRRSV and H. parasuis decrease production of ROS indicating the presence of unspecified defence mechanism used by H. parasuis to defend itself against respiratory burst. Obtained data showed that simultaneous infection of PAMs with PRRSV and H. parasuis elicits strong pro-inflammatory immune response and that H. parasuis can probably protect itself against respiratory stress. Severe disease in natural conditions caused by both pathogens could be therefore consequence of pro-inflammatory mediated immunopathology. The study was supported by the Ministry of Agriculture of the Czech Republic (MZE0002716202, QJ1210120) and project AdmireVet (CZ 1.05/2.1.00/01.0006; ED0006/01/01).