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Effect of bovine lactoferrin on IPEC infected by porcine rotavirus A

Název česky Vliv bovinního laktoferinu na buněčnou linii IPEC infikovanou porcinním rotavirem A


Rok publikování 2013
Druh Konferenční abstrakty
Fakulta / Pracoviště MU

Přírodovědecká fakulta

Popis Lactoferrin is an iron-binding glycoprotein which displays many activities including antiviral one. Lactoferrin is thought to have protective effects against rotaviral enteral infection. Rotaviruses are dsRNA viruses in the family Reoviridae. Group A rotaviruses (RVAs) are a major cause of acute gastroenteritis in young children and in a wide variety of domestic animals, including pigs. Propagation of both human and animal rotavirus strains requires proteolytic activation with enzyme trypsin prior to infection. Our intention was to construct growth curves of RVA strain OSU CAPM V-334 (obtained from Collection of animal pathogenic microorganisms, Brno, Czech Republic) on IPEC cells. Growth curve of rotavirus was monitored for two days in two-hour intervals. Infection was terminated by freezing in -80°C. Samples were analyzed using qRT-PCR to assess the number of RVA genome copies. Data obtained from the growth curve was used in the following experiment. Eight experimental variants were used: IPEC; IPEC and trypsin; IPEC and trypsin and rotavirus; IPEC and rotavirus, IPEC and trypsin and lactoferrin, IPEC and lactoferrin, IPEC and trypsin and lactoferrin and rotavirus; IPEC and lactoferrin and rotavirus. In particular variants, IPEC were incubated for 1 hour with lactoferrin (100 µg/ml), and then thereto a viral infection was added for 20 min. The cells were then sealed with maintenance medium and incubated for 6 hours. We monitored the level of expression of IL-6 using qRT-PCR. The rate of expression of IL-6 was significantly increased in cells infected with rotavirus, compared to uninfected cells. At the same time there was no significant effect of lactoferrin on IL-6 gene expression. The study was supported by projects AdmireVet CZ.1.05/2.1.00/01.006 (ED006/01/01) and by the Ministry of Agriculture of the Czech Republic (QJ1310258)

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