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Seasonal variation in alkaloid composition and antiproliferative activity of Stylophorum lasiocarpum (Oliv.) Fedde

Název česky Sezonní změny v obsahu alkaloidů a stanovení antiproliferační aktivity Stylophorum lasiocarpum
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ŠEBRLOVÁ Kristýna PEŠ Ondřej SLANINOVÁ Iva VYMAZAL Ondřej KANTOROVÁ Jana TÁBORSKÁ Eva

Rok publikování 2015
Druh Článek v odborném periodiku
Časopis / Zdroj Chemical papers
Fakulta / Pracoviště MU

Lékařská fakulta

Citace
www http://www.chemicalpapers.com/?id=7&paper=8475
Doi http://dx.doi.org/10.1515/chempap-2015-0083
Obor Biochemie
Klíčová slova Stylophorum lasiocarpum; traditional Chinese medicine; vegetation period; benzophenanthridine; LC MS; cytotoxicity
Popis Stylophorum lasiocarpum (Oliv.) Fedde (Papaveraceae) belongs to traditional Chinese medicine herbs but there was minimal information on the content of alkaloids in this plant. Extracts from the aerial part and roots were examined by liquid chromatography with UV and mass spectrometric detection, with nineteen alkaloids identified. Changes in alkaloid content over the entire vegetation period of a one- and two-year old plant were studied. The protoberberine alkaloids, coptisine and stylopine, were found to be the main substances in extracts of the aerial part irrespective of the plant’s age and time of harvest. Variable amounts of protopine, sanguinarine, chelerythrine, chelirubine, macarpine, chelilutine and berberine were also recorded in the aerial part. The roots contained significantly larger quantities of all alkaloids than the aerial part with the levels of most alkaloids varying from May to October, peaking in the middle of the vegetation period. Coptisine was the dominant alkaloid in all samples. The antiproliferative activities of the root extract and of seven individual alkaloids were tested on A375 human malignant melanoma cells. The significant dose-dependent toxicity of the root extract was attributed largely to the quaternary benzo[c]phenanthridine alkaloids, macarpine and sanguinarine.
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