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Localized movement and morphology of UBF1-positive nucleolar regions are changed by gamma-irradiation in G2 phase of the cell cycle

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SOROKIN Dmitry STIXOVÁ Lenka SEHNALOVÁ Petra LEGARTOVÁ Soňa SUCHÁNKOVÁ Jana ŠIMARA Pavel KOZUBEK Stanislav MATULA Pavel SKALNÍKOVÁ Magdalena RAŠKA Ivan BÁRTOVÁ Eva

Druh Článek v odborném periodiku
Časopis / Zdroj Nucleus
Fakulta / Pracoviště MU

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Citace
Doi http://dx.doi.org/10.1080/19491034.2015.1075111
Obor Genetika a molekulární biologie
Klíčová slova DNA damage; live cells; nucleolus; uncleoli tracking; UBF1
Popis The nucleolus is a well-organized site of ribosomal gene transcription. Moreover, many DNA repair pathway proteins, including ATM, ATR kinases, MRE11, PARP1 and Ku70/80, localize to the nucleolus (Moore et al., 2011). We analyzed the consequences of DNA damage in nucleoli following ultraviolet A (UVA), C (UVC), or g-irradiation in order to test whether and how radiation-mediated genome injury affects local motion and morphology of nucleoli. Because exposure to radiation sources can induce changes in the pattern of UBF1-positive nucleolar regions, we visualized nucleoli in living cells by GFP-UBF1 expression for subsequent morphological analyses and local motion studies. UVA radiation, but not 5 Gy of g-rays, induced apoptosis as analyzed by an advanced computational method. In non-apoptotic cells, we observed that gradiation caused nucleolar re-positioning over time and changed several morphological parameters, including the size of the nucleolus and the area of individual UBF1-positive foci. Radiation-induced nucleoli rearrangement was observed particularly in G2 phase of the cell cycle, indicating repair of ribosomal genes in G2 phase and implying that nucleoli are less stable, thus sensitive to radiation, in G2 phase.
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