Informace o publikaci

Post-Translational Modifications of Histones in Human Sperm

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KREJČÍ Jana STIXOVÁ Lenka PAGÁČOVÁ Eva LEGARTOVÁ Soňa KOZUBEK Stanislav LOCHMANOVÁ Gabriela ZDRÁHAL Zbyněk SEHNALOVÁ Petra DABRAVOLSKI Siarhei HEJÁTKO Jan BÁRTOVÁ Eva

Rok publikování 2015
Druh Článek v odborném periodiku
Časopis / Zdroj Journal of cellular biochemistry
Fakulta / Pracoviště MU

Středoevropský technologický institut

Citace
www http://onlinelibrary.wiley.com/doi/10.1002/jcb.25170/pdf
Doi http://dx.doi.org/10.1002/jcb.25170
Obor Genetika a molekulární biologie
Klíčová slova HUMAN SPERM; HISTONES; PROTAMINE P2; EPIGENETICS; MICROSCOPY
Popis We examined the levels and distribution of post-translationally modified histones and protamines in human sperm. Using western blot immunoassay, immunofluorescence, mass spectrometry (MS), and FLIM-FRET approaches, we analyzed the status of histone modifications and the protamine P2. Among individual samples, we observed variability in the levels of H3K9me1, H3K9me2, H3K27me3, H3K36me3, and H3K79me1, but the level of acetylated (ac) histones H4 was relatively stable in the sperm head fractions, as demonstrated by western blot analysis. Sperm heads with lower levels of P2 exhibited lower levels of H3K9ac, H3K9me1, H3K27me3, H3K36me3, and H3K79me1. A very strong correlation was observed between the levels of P2 and H3K9me2. FLIM-FRET analysis additionally revealed that acetylated histones H4 are not only parts of sperm chromatin but also appear in a non-integrated form. Intriguingly, H4ac and H3K27me3 were detected in sperm tail fractions via western blot analysis. An appearance of specific histone H3 and H4 acetylation and H3 methylation in sperm tail fractions was also confirmed by both LC-MS/MS and MALDI-TOF MS analysis. Taken together, these data indicate that particular post-translational modifications of histones are uniquely distributed in human sperm, and this distribution varies among individuals and among the sperm of a single individual.
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