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In-vivo Fluorescence Macroscopy in Plants

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PERNISOVÁ Markéta HEJÁTKO Jan

Rok publikování 2014
Druh Vyžádané přednášky
Fakulta / Pracoviště MU

Středoevropský technologický institut

Citace
Popis Many microscopic techniques exist in plant biology and microscopic slides are necessary to use usually. Together with Nikon company we developed horizontally oriented confocal macroscope for in-vivo plant imaging on plates directly. Optical system with objectives is horizontally oriented and thus plants can grow vertically similarly to real conditions in soil. This setup allows observing plant’s biological processes in a real time, for example protein relocalization, changes in metabolite concentration, cell elongation and division or root growth. No transmitted light is a small disadvantage but it is possible to use plant lines with fluorescent protein labelled plasma membranes. Taken together, horizontal confocal macroscope is very useful instrument for real-time imaging of growing plants.
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