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Complete genome analysis of ETA-converting bacteriophages isolated from impetigo strains of Staphylococcus aureus

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BOTKA Tibor RŮŽIČKOVÁ Vladislava KONEČNÁ Hana PANTŮČEK Roman RYCHLÍK Ivan ZDRÁHAL Zbyněk PETRÁŠ Petr DOŠKAŘ Jiří

Rok publikování 2015
Druh Článek ve sborníku
Konference Týden lékařské genetiky v Brně - The Biomania Student Scientific Meeting 2015
Fakulta / Pracoviště MU

Přírodovědecká fakulta

Citace
www The Biomania Student Scientific Meeting 2015
Obor Genetika a molekulární biologie
Klíčová slova ETA phage, Exfoliative toxin A, structure proteins spectre, genome analysis
Popis Exfoliative toxin A (ETA)-coding temperate bacteriophages are the main contributors to the toxic phenotype of impetigo strains of Staphylococcus aureus. Two eta gene-positive phages isolated from S. aureus strains which recently caused massive outbreaks of pemphigus neonatorum in several Czech maternity hospitals were characterized in detail to elucidate their genome diversity. The phages, designated phiB166 and phiB236, were able to transfer the eta gene into a prophageless S. aureus strain which afterwards converted into an ETA producer. Complete phage genome sequences were determined and described. In addition, promoters and intrinsic terminators were predicted. With respect to the functional genomic architecture, the genomes were divided into five regions. The comparative analysis revealed major variances between the phages. They differed in the genome size, number of open reading frames (ORFs) and genome architecture. High mutual sequence similarity was detected only in the terminal region. Proteomic analysis showed differences in virion protein patterns. When compared with the so far described eta and non eta phages, noticeable differences were found. Sequences gained by possible recombination were identified in genomes of both phages. Thus, these phages represent two new types of as yet not characterized bacteriophages of the Siphoviridae family having impact on pathogenicity of impetigo strains of S. aureus.
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