Informace o publikaci

Introduction of macarpine as a novel cell-permeant DNA dye for live cell imaging and flow cytometry sorting

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SLANINOVÁ Iva LÓPEZ-SÁNCHEZ Noelia ŠEBRLOVÁ Kristýna VYMAZAL Ondřej FRADE José María TÁBORSKÁ Eva

Rok publikování 2016
Druh Článek v odborném periodiku
Časopis / Zdroj Biology of the Cell
Fakulta / Pracoviště MU

Lékařská fakulta

Citace
Doi http://dx.doi.org/10.1111/boc.201500047
Obor Genetika a molekulární biologie
Klíčová slova Cell cycle; DNA dye; FACS; Macarpine; Time-lapse imaging
Popis Macarpine (MA) is a quaternary benzophenanthridine plant alkaloid isolated from Macleaya microcarpa or Stylophorum lasiocarpum. Benzophenanthridine alkaloids are interesting natural products that display antiproliferative, antimicrobial, antifungal and anti-inflammatory activities, and also fluorescence properties. In a previous study, we demonstrated that thanks to its ability to interact with DNA and its spectral properties MA could be used as a supravital DNA probe for fluorescence microscopy and flow cytometry including analyses of the cell cycle. In this study, we evaluated the suitability of MA as a DNA dye for time-lapse microscopy and flow-cytometric cell sorting.
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