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The E3 ubiquitin ligase Mib1 regulates Plk4 and centriole biogenesis

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ČAJÁNEK Lukáš GLATTER Timo NIGG Erich A.

Rok publikování 2015
Druh Článek v odborném periodiku
Časopis / Zdroj Journal of Cell Science
Fakulta / Pracoviště MU

Lékařská fakulta

Citace
Doi http://dx.doi.org/10.1242/jcs.166496
Obor Genetika a molekulární biologie
Klíčová slova Cell cycle; Centriole; Centrosome; Kinase; Ubiquitin; Mib4; Plk4
Popis Centrioles function as core components of centrosomes and as basal bodies for the formation of cilia and flagella. Thus, effective control of centriole numbers is essential for embryogenesis, tissue homeostasis and genome stability. In mammalian cells, the centriole duplication cycle is governed by Polo-like kinase 4 (Plk4). Here, we identify the E3 ubiquitin ligase Mind bomb (Mib1) as a new interaction partner of Plk4. We show that Mib1 localizes to centriolar satellites but redistributes to centrioles in response to conditions that induce centriole amplification. The E3 ligase activity of Mib1 triggers ubiquitylation of Plk4 on multiple sites, causing the formation of Lys11-, Lys29- and Lys48-ubiquitin linkages. These modifications control the abundance of Plk4 and its ability to interact with centrosomal proteins, thus counteracting centriole amplification induced by excess Plk4. Collectively, these results identify the interaction between Mib1 and Plk4 as a new and important element in the control of centriole homeostasis.
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