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A modern molecular approach in food safety and food-borne parasitoses

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RESLOVÁ Nikol KAŠNÝ Martin SLANÝ Michal KRÁLÍK Petr

Druh Další prezentace na konferencích
Fakulta / Pracoviště MU

Přírodovědecká fakulta

Citace
Popis In recent years, there has been an increase in the numbers of reported outbreaks, cases, associated with food-borne parasitic infections arising from meat consumption. This trend is affected by the changes in farming practices towards bio-production, globalization of food market, global climate change and also by the way of meat processing. All these facts facilitate the risks of widespread disseminations of food-borne diseases, including the pathogens of protozoa and multi-cellular parasites such as Toxoplasma gondii, Trichinella spiralis or Taenia saginata, and consequently induce the need of improvement of the various diagnostic tools for food-borne agents in the final food products such as meat. In relation to this issue, our work is focused on development of reliable comprehensive molecular diagnostic method useful for rapid control of meat products on the market. For this purpose, we adopted high sensitive multiplex oligonucleotide ligation- PCR assay - MOL-PCR, enabling direct and simultaneous detection of multiple nucleic acid signatures from complex mixtures potentially containing the DNA originating from number of different parasitic organisms and xMAP technology representing the novel platform based on magnetic microspheres. Visualization of the products is then realized via MAGPIX instrument for qualitative and quantitative readout of signal. Up to this date, the specific molecular probes allowing the detection and capturing of targeted DNA from two parasitic worms - Trichinella spiralis (partial sequence of 18S rRNA gene) and Taenia saginata (partial sequence of mitochondrial COX1 gene) were designed. Reaction conditions of MOL-PCR were optimized for simultaneous duplex assay. Currently, the calibration of MAGPIX system is in process together with arranging procedure for preparing the sample – from election of the most suitable input material to DNA isolation.
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