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Immune response of porcine alveolar macrophages to a concurrent infection with porcine reproductive and respiratory syndrome virus and Haemophilus parasuis in vitro

Název česky Imunitní odpověď prasečích alveolárních makrofágů na souběžnou infekci virem prasečího reprodukčního a respiračního syndromu a Haemophilem parasuis in vitro
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KAVANOVÁ Lenka PRODĚLALOVÁ Jana NEDBALCOVÁ Kateřina MATIAŠOVIC Ján VOLF Jiří FALDYNA Martin SALÁT Jiří

Druh Článek v odborném periodiku
Časopis / Zdroj Veterinary Microbiology
Fakulta / Pracoviště MU

Přírodovědecká fakulta

Citace
www http://www.sciencedirect.com/science/article/pii/S0378113515300158
Doi http://dx.doi.org/10.1016/j.vetmic.2015.08.026
Obor Imunologie
Klíčová slova Haemophilus parasuis; IL-1ß; PRRSV; Porcine alveolar macrophages; Reactive oxygen species
Popis Porcine reproductive and respiratory syndrome virus (PRRSV) can predispose pigs to secondary respiratory infection with bacteria such as Haemophilus parasuis. Animals infected with both pathogens develop more severe clinical disease. The immune response of porcine alveolar macrophages (PAMs) to simultaneous infection with PRRSV and H. parasuis was analysed in vitro, describing cytokine production, expression of cell surface molecules, and production of reactive oxygen species (ROS). Concurrent infection with PRRSV and H. parasuis increased gene expression of pro-inflammatory cytokines (TNF-?, IL-1ß, IL-8) in PAMs in comparison with PAMs infected with PRRSV or H. parasuis alone. An additive effect of dual infection on IL-1ß production was confirmed at the protein level. PAMs infected with PRRSV showed increased production of ROS compared to controls. Conversely, simultaneous infection of PAMs with PRRSV and H. parasuis decreased production of ROS, indicating the presence of an H. parasuis defence mechanism against respiratory burst. Concurrent infection of PAMs with PRRSV and H. parasuis was shown to elicit a pro-inflammatory immune response represented by significant IL-1ß production. Severe multifactorial respiratory disease in natural conditions caused by both pathogens could be the consequence of pro-inflammatory mediated immunopathology.