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TUT-DIS3L2 is a mammalian surveillance pathway for aberrant structured non-coding RNAs

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USTIANENKO Dmytro PASULKA Josef FEKETOVÁ Zuzana BEDNAŘÍK Lukáš ZIGÁČKOVÁ Dagmar FOŘTOVÁ Andrea ZAVOLAN Mihaela VAŇÁČOVÁ Štěpánka

Druh Článek v odborném periodiku
Časopis / Zdroj EMBO JOURNAL
Fakulta / Pracoviště MU

Středoevropský technologický institut

Citace
www http://emboj.embopress.org/content/early/2016/09/19/embj.201694857
Doi http://dx.doi.org/10.15252/embj.201694857
Obor Biochemie
Klíčová slova DIS3L2; RNA surveillance; TSSa; ncRNAs; uridylation
Popis Uridylation of various cellular RNA species at the 3' end has been generally linked to RNA degradation. In mammals, uridylated pre-let-7 miRNAs and mRNAs are targeted by the 3' to 5' exoribonuclease DIS3L2. Mutations in DIS3L2 have been associated with Perlman syndrome and with Wilms tumor susceptibility. Using in vivo cross-linking and immunoprecipitation (CLIP) method, we discovered the DIS3L2-dependent cytoplasmic uridylome of human cells. We found a broad spectrum of uridylated RNAs including rRNAs, snRNAs, snoRNAs, tRNAs, vault, 7SL, Y RNAs, mRNAs, lncRNAs, and transcripts from pseudogenes. The unifying features of most of these identified RNAs are aberrant processing and the presence of stable secondary structures. Most importantly, we demonstrate that uridylation mediates DIS3L2 degradation of short RNA polymerase II-derived RNAs. Our findings establish the role of DIS3L2 and oligouridylation as the cytoplasmic quality control for highly structured ncRNAs.
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