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Identification of miRNA expression profiles in human mesial temporal lobe epilepsy with hippocampal sclerosis

Název česky Identifikace miRNA profilů v lidské meziotemporální epilepsií s hipokampální sklerózou
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BENCÚROVÁ Petra BALOUN Jiří MUSILOVÁ Kateřina PÁL Karol TOM Nikola HYNŠT Jakub RADOVÁ Lenka PAIL Martin BRICHTOVÁ Eva HERMANOVÁ Markéta POSPÍŠILOVÁ Šárka MRÁZ Marek BRÁZDIL Milan

Rok publikování 2016
Druh Konferenční abstrakty
Fakulta / Pracoviště MU

Středoevropský technologický institut

Citace
Popis Purpose: The primary aim of this study was to identify specific miRNAs with aberrant expression in hippocampal tissue of mesial temporal lobe epilepsy (mTLE) patients with hippocampal sclerosis (HS). Method: We analyzed miRNA isolated from paraffin embedded tissue slices of hippocampal complex from 22 mTLE +HS patients (14 left-sided, 8 right-sided) and 9 post mortem controls. To characterize the miRNAs highly prone to be involved in the pathological changes in the mTLE+HS brain tissue we performed the expression analysis combining the next generation sequencing (NGS) and novel specific technique for miRNA quantification miQPCR. Results: By both methodical approaches we confirmed significant upregulation of miR-129-1-3p, 129-2-3p, 142-3p, 191-5p, 193b-3p, 195-5p, 374b-5p, 451a, and 490-3p in mTLE+HS patients. The levels of miRNA-184 were significantly higher in control subjects. Differential expression levels in mTLE+ HS patients were identified also in the case of 10 miRNA species never considered to play a role in epilepsy before. Conclusion: Analytical approach combining NGS with miQPCR used for the first time for analysis of human epileptic foci led to reliable identification of miRNA with altered expression in mTLE +HS patients.
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