Informace o publikaci
Chloroacetamide-Linked Nucleotides and DNA for Cross-Linking with Peptides and Proteins
| Autoři | |
|---|---|
| Rok publikování | 2016 |
| Druh | Článek v odborném periodiku |
| Časopis / Zdroj | BIOCONJUGATE CHEMISTRY |
| Fakulta / Pracoviště MU | |
| Citace | |
| www | http://pubs.acs.org/doi/abs/10.1021/acs.bioconjchem.6b00342 |
| Doi | http://dx.doi.org/10.1021/acs.bioconjchem.6b00342 |
| Obor | Biochemie |
| Klíčová slova | TUMOR-SUPPRESSOR P53; DIELS-ALDER REACTION; REDUCTIVE AMINATION; ENZYMATIC-SYNTHESIS; LYSINE RESIDUES; CLICK CHEMISTRY; BINDING; TRIPHOSPHATES; PROBES; AZIDE |
| Popis | Nucleotides, 2'-deoxyribonucleoside triphosphates (dNTPs), and DNA probes bearing reactive chloroacetamido group linked to nucleobase (cytosine or 7-deazadaenine) through a propargyl tether were prepared and tested in cross-linking with cysteine- or histidine-containing peptides and proteins. The chloroacetamide-modifed dNTPs proved to be good substrates for DNA polymerases in the enzymatic synthesis of modified DNA probes. Modified nucleotides and DNA reacted efficiently with cysteine and cysteine-containing peptides, whereas the reaction with histidine was sluggish and low yielding. The modified DNA efficiently cross-linked with p53 protein through alkylation of cysteine and showed potential for cross-linking with histidine (in C277H mutant of p53). |
| Související projekty: |