Informace o publikaci

Chloroacetamide-Linked Nucleotides and DNA for Cross-Linking with Peptides and Proteins

Autoři

OLSZEWSKA Agata POHL Radek BRAZDOVA Marie FOJTA Miroslav HOCEK Michal

Rok publikování 2016
Druh Článek v odborném periodiku
Časopis / Zdroj BIOCONJUGATE CHEMISTRY
Fakulta / Pracoviště MU

Středoevropský technologický institut

Citace
www http://pubs.acs.org/doi/abs/10.1021/acs.bioconjchem.6b00342
Doi http://dx.doi.org/10.1021/acs.bioconjchem.6b00342
Obor Biochemie
Klíčová slova TUMOR-SUPPRESSOR P53; DIELS-ALDER REACTION; REDUCTIVE AMINATION; ENZYMATIC-SYNTHESIS; LYSINE RESIDUES; CLICK CHEMISTRY; BINDING; TRIPHOSPHATES; PROBES; AZIDE
Popis Nucleotides, 2'-deoxyribonucleoside triphosphates (dNTPs), and DNA probes bearing reactive chloroacetamido group linked to nucleobase (cytosine or 7-deazadaenine) through a propargyl tether were prepared and tested in cross-linking with cysteine- or histidine-containing peptides and proteins. The chloroacetamide-modifed dNTPs proved to be good substrates for DNA polymerases in the enzymatic synthesis of modified DNA probes. Modified nucleotides and DNA reacted efficiently with cysteine and cysteine-containing peptides, whereas the reaction with histidine was sluggish and low yielding. The modified DNA efficiently cross-linked with p53 protein through alkylation of cysteine and showed potential for cross-linking with histidine (in C277H mutant of p53).
Související projekty:

Používáte starou verzi internetového prohlížeče. Doporučujeme aktualizovat Váš prohlížeč na nejnovější verzi.

Další info