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Fast and Reliable Differentiation of Eight Trichinella Species Using a High Resolution Melting Assay

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RESLOVÁ Nikol ŠKORPÍKOVÁ Lucie SLANÝ Michal POZIO Edoardo KAŠNÝ Martin

Druh Článek v odborném periodiku
Časopis / Zdroj Scientific Reports
Fakulta / Pracoviště MU

Přírodovědecká fakulta

Citace
WWW https://www.nature.com/articles/s41598-017-16329-x
Doi http://dx.doi.org/10.1038/s41598-017-16329-x
Obor Zoologie
Klíčová slova qPCR-HRMA; Trichinella; species identification; COI; ESV
Přiložené soubory
Popis High resolution melting analysis (HRMA) is a single-tube method, which can be carried out rapidly as an additional step following real-time quantitative PCR (qPCR). The method enables the differentiation of genetic variation (down to single nucleotide polymorphisms) in amplified DNA fragments without sequencing. HRMA has previously been adopted to determine variability in the amplified genes of a number of organisms. However, only one work to date has focused on pathogenic parasites–nematodes from the genus Trichinella. In this study, we employed a qPCR-HRMA assay specifically targeting two sequential gene fragments–cytochrome c oxidase subunit I (COI) and expansion segment V (ESV), in order to differentiate 37 single L1 muscle larvae samples of eight Trichinella species. We prove that qPCR-HRMA based on the mitochondrial COI gene allows differentiation between the sequences of PCR products of the same length. This simple, rapid and reliable method can be used to identify at the species level single larvae of eight Trichinella taxa.
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