Informace o publikaci

Heavy water enhancement of fluorescence signal in reversed-phase liquid chromatography

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TÁBORSKÝ Petr KUČERA Josef JUŘICA Jan PEŠ Ondřej

Rok publikování 2018
Druh Článek v odborném periodiku
Časopis / Zdroj JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
Fakulta / Pracoviště MU

Lékařská fakulta

Citace
www https://www.sciencedirect.com/science/article/pii/S157002321830669X
Doi http://dx.doi.org/10.1016/j.jchromb.2018.05.037
Klíčová slova Liquid chromatography; Heavy water; Fluorescence; Deuterium; Mass spectrometry; Doxorubicin; Fluorescein; Vitamin B2
Popis Liquid chromatography with fluorescence detection has been used in analyses demanding high sensitivity and selectivity. As majority systems rely on reversed-phase columns with water being the main component of the mobile phase, fluorescent compounds with emission maxima higher than 500 nm might be dynamically quenched. A simple replacement of H2O with D2O enhanced the sensitivity for selected compounds by 10–200 per cent. Affected compounds included an anti-cancer drug doxorubicin, a luminescent probe fluorescein, and naturally occurring forms of vitamin B2. Similar levels of enhancement were obtained by fluorescence spectrometry. Such simple yet effective approach may greatly improve HPLC analyses coupled to fluorescence detection.
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