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Molecular detection of Toxoplasma gondii in feathered game intended for human consumption in the Czech Republic

Název česky Molekulární detekce Toxoplasma gondii u pernaté zvěře určené k lidské spotřebě v České republice
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ŠKORPÍKOVÁ Lucie RESLOVÁ Nikol LORENCOVÁ Alena PLHAL Radim DRIMAJ Jakub KAMLER Jiří SLANÝ Michal

Druh Článek v odborném periodiku
Časopis / Zdroj International Journal of Food Microbiology
Fakulta / Pracoviště MU

Přírodovědecká fakulta

Citace
WWW https://www.sciencedirect.com/science/article/pii/S0168160518303763?via%3Dihub
Doi http://dx.doi.org/10.1016/j.ijfoodmicro.2018.07.019
Klíčová slova Toxoplasmosis; Food safety; Real-time PCR; Genotyping; Ducks; Pheasants
Popis Toxoplasma gondii is an important ubiquitous protozoan parasite, which can infect almost all warm-blooded vertebrates, including humans. The diagnosis of T. gondii infection is crucial for the prevention, surveillance, and control of its transmission. Here, a triplex real-time PCR assay targeting the B1 gene and 529rep element was used to determine the presence of T. gondii in feathered game (Anas platyrhynchos and Phasianus colchicus) hunted in the Czech Republic. The prevalence of T. gondii was 5.4% in wild ducks (n = 280) and 3.4% in common pheasants (n = 350). Additionally, genotyping of 28 T. gondii-positive samples revealed the presence of archetypal genotypes II and III as well as non-archetypal genotypes combining both type II and III alleles. Our results suggest that consumption of feathered game could pose a risk of T. gondii transmission to humans in the Czech Republic.