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Interactions of blood-feeding monogenean parasite with the fish host immune system

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ILGOVÁ Jana SALÁT Jiří GELNAR Milan KAŠNÝ Martin

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Fakulta / Pracoviště MU

Přírodovědecká fakulta

Citace
Popis Parasites use wide repertoire of molecules to manipulate the host’s immune system. Such interaction ensures the increased chances for parasite survival and reproduction. Nevertheless, the molecular communication between the fish host and parasitic worms from the group Monogenea have been topic omitted by the research. We chose experimental host-parasite system – blood-feeding, ectoparasitic Eudiplozoon nipponicum (Monogenea: Diplozoidae) and its typical host – common carp (Cyprinus carpio) and identified multiple parasite’s molecules with immunomodulatory potential. We selected cysteine peptidase inhibitor, also called type I cystatin or stefin (EnStef), which we recognized as relatively highly expressed, for further immune assays. After in vitro incubation of fish macrophages with recombinant EnStef we analysed the expression levels of pro- and anti-inflammatory cytokines (TNF-alpha, IL1-beta, IL-6, IL-8, IL-10) produced by the cells. Our preliminary results suggest that EnStef may cause down-regulation of studied cytokines.
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