Informace o publikaci

Magnet Activated Cell Sorting Method in Sperm Selection and Their Benefit in Human Assisted Reproduction

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VENTRUBA Pavel JEŠETA Michal ŽÁKOVÁ Jana KEMPISTY B. CHMELÍKOVÁ Eva CRHA Igor

Rok publikování 2018
Druh Článek v odborném periodiku
Časopis / Zdroj Biomedical journal of scientific & technical research
Fakulta / Pracoviště MU

Lékařská fakulta

Citace
Doi http://dx.doi.org/10.26717/BJSTR.2018.06.001378
Klíčová slova Spermatozoa; MACS; DNA integrity;Swim-up;Apoptosis; Sperm preparation
Popis Center of Assisted Reproduction in University Hospital Brno is the oldest one in Czech Republic and is linked to academic institution, Masaryk University Brno. We have long time experiences with the therapy of human infertility. During last years we are focused on evaluation of novel techniques in assisted reproduction. Sperm selection for assisted reproductive techniques (ART) is very important step for successful in vitro fertilization [l-6].Classical ICSI (introcytoplasmatic sperm injection) method is not optimal for subjective sperm selection based on only morphology and motility of spermatozoa. For this reason there is currently a trend to combine multiple methods to segregate inappropriate sperm based on multiple parameters to increase the likelihood of successful fertilization. Is it usually classical separation method like swim-up or density gradient and a group of additional techniques like PICSI (ICSI with preselected sperm). One of these additional methods is MACS (magnet activated cell sorting). How many methods are best combined, and whether a new MACS selection method really brings a better prognosis in terms of conception success, or from the point of view of the subsequent development of the foetus, has already been studied several times.
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