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Radiofrequency ablation and transarterial chemoembolisation are characterized by changing dynamics of circulating microRNAs

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ZAVADIL Jan ANDRAŠINA Tomáš JURÁČEK Jan ČECHOVÁ Barbora SLABÝ Ondřej GOLDBERG Nahum

Druh Konferenční abstrakty
Fakulta / Pracoviště MU

Lékařská fakulta

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Popis Purpose To investigate whether the expression level of circulating microRNAs related to hypoxia (miR-21 and miR-210), liver injury (miR-122) and epithelial-mesenchymal transition (miR-200a) could reflect changes in patients who underwent thermal ablation and chemoembolization. Material and methods This prospective study consisted of 30 patients diagnosed with hepatocellular carcinoma and liver metastases of colorectal cancer treated with thermal ablation (n=17) and chemoembolization (n=13). Thermal ablation was performed using a radiofrequency or microwave generator (RITA, Microsulis, AngioDynamics,Inc). For TACE, drug eluting beads (DCBeads, Biocompatibles Ltd.) were used. The tumour burden and treatment response was evaluated by RECIST, mRECIST and volumetry analyses. Four blood samples (before intervention, immediately after intervention, 24 hours after intervention, and control sample 1 week after intervention) were taken to measure plasmatic concentration of miRNA using miRNA-specific TaqMan assays and qRT-PCR method. Results In thermal ablation cases we observed a significant increase in investigated miRNA concentrations immediately after intervention (miR-122, FC=15, miR-200a, FC=1.9, P<0.05). In TACE, we observed a delayed increase in circulating miRNA concentrations 24 hours after intervention (miR-21, FC=10.4, miR-210, FC=9.0, miR-122, FC=27, miR-200a, FC=4.0, P<0.05). In both methods, the initial increase was followed by a steady decline of miRNA levels. Identified dynamic changes in circulating miRNA levels were in accordance with the nature of the thermal ablation and TACE hypoxia-related biological effects.The significant bias of tumour burden or treatment response was not shown in this sample size. Conclusion Our preliminary data indicates potential usage of circulating miRNAs for monitoring of the systemic effects of thermal ablative and intraarterial therapies.
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